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Cell line: | MONO-MAC-1 |
DSMZ no.: | ACC 252 |
Species: | human (Homo sapiens) |
Cell type: | acute monocytic leukemia |
Origin: | established from the peripheral blood of a 64-year-old man with acute monocytic leukemia (AML FAB M5) at relapse in 1985 following myeloid metaplasia; simultaneous sister cell line of MONO-MAC-6 (DSM ACC 124); cells are described in the literature to be less differentiated than MONO-MAC-6 and to support productive infection with HIV-1; see also website "www.monocyte.eu". |
Reference(s): | 15078, 14554, 14986 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Commercial entities need a permit from the original investigator (please contact DSMZ: mutz@dsmz.de)).Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | single, round/multiformed cells in suspension; image; image; image;image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine + 1x non-essential amino acids + 1 mM sodium pyruvate |
Subculture: | seed out at ca. 1 x 106 cells/ml; maintain at 0.3-1.0 x 106 cells/ml; split ratio 1:2 to 1:3 every 2-3 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 30-50 hours |
Harvest: | maximal density at 1.0-1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | CD3 -, CD13 +, CD14 -, CD15 +, CD19 -, CD34 -, cyCD68 +, HLA-DR +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near-diploid karyotype with 10% polyploidy - 49(43-52)<2n>XY, +8, +12, +13, +18, -20, inv dup(3)(q21q27), add(8)(q24), t(9;11)(p22;q23), t(10;12;17)(q24;q13;q11), r(12)(??), del(13)(q1?2q21), t(16;21)(q13;q22.2) - diploid version of its sister MONO-MAC-6 with fewer in vitro rearrangements - carries 9;11 translocation (AML M5) |
Molec. Genetics: | expression of fusion gene KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) confirmed by RT-PCR |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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