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Cell line: | HUP-T3 |
DSMZ no.: | ACC 259 |
Species: | human (Homo sapiens) |
Cell type: | pancreas carcinoma |
Origin: | established in 1984 from the malignant ascites of a 66-year-old man with a poorly differentiated adenocarcinoma of the body and tail of the pancreas; described to be tumorgenic in nude mice |
Reference(s): | 14808, 40710 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epitheloid cells growing adherently in monolayers and clusters; image; image; image |
Medium: | 90% MEM (with Earle's salts) + 10% h.i. FBS + 1 mM non-essential amino acids + 1 mM sodium pyruvate |
Subculture: | seed out about 1 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 3-4 days
using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 70 hours |
Harvest: | about 14 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hypodiploid karyotype with 13% polyploidy - 39(36-40)<2n>XY, -6, -8, -9, -10, -12, -13, -17, -17, -19, -20, +3mar (2 rings), del(4)(p15), add(11)(q22.3;HSR), add(12)(q24;HSR), add(19)(p13), add(21)(p12) - gene amplification suggested by large HSR - ch 17 nullisomy |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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