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Cell line: | WEHI-3B |
DSMZ no.: | ACC 26 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | myelomonocytic leukemia |
Origin: | macrophage-like cells derived from a BALB/c mouse treated for tumor induction; cells produce IL-3 |
Reference(s): | 14877, 14878, 15054, 15222, 18195 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | small, round, single cells growing adherently and partly in suspension; image; image; image; image; image |
Medium: | 90-95% Iscove's MDM + 5-10% h.i. FBS + 2 mM L-glutamine |
Subculture: | split confluent culture 1:2 to 1:5 twice to three times weeky; cells are trypsin-sensitive, detach mechanically after loosening by incubating in PBS (with Ca2+ or Mg2+) for 10-15 min; seed out at ca. 0.2-0.5 x 105 cells/cm2; alternatively, cells can be detached using TrypLE Express (Invitrogen) |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 20-30 hours |
Harvest: | maximum cell harvest of about 1 x 106 cells/cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Cytogenetics: | murine hypotetraploid karyotype with 19% polyploidy - 73/74(70-79)<4n> - 2-3 centric fusion markers present |
Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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