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| Cell line: | RGE |
| DSMZ no.: | ACC 262 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | endothelial glomerular kidney |
| Origin: | established from freshly isolated glomeruli from kidneys of 6-week-old female Sprague-Dawley rats |
| Reference(s): | 14707 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent epitheloid cells growing as monolayer (after confluency cells tend to grow in multilayers); image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | split confluent culture 1:2 to 1:3 twice a week using trypsin/EDTA (cells are only lightly adherent and can be detached by tapping the flask; however, to prepare single cell suspension, trypsin/EDTA treatment is necessary); seed out at ca. 2 x 106 cells/80 cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 17 hours |
| Harvest: | cell harvest of about 40-50 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Cytogenetics: | rat hypertriploid karyotype with 6% polyploidy - 67(67-72)<3n> - corresponds to published karyotype |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
