| | |
|---|
| Cell line: | S-117 |
| DSMZ no.: | ACC 266 |
| Species: | human (Homo sapiens) |
| Cell type: | thyroid sarcoma |
| Origin: | established from the sarcoma of the thyroid gland from a 47-year-old woman in 1985 |
| Reference(s): | 15139 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid cells growing adherently as monolayer; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | split confluent culture 1:2 about once a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 37 hours |
| Harvest: | cell harvest of about 0.5-1.0 x 105 cells/cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hexaploid/octaploid karyotype with 14% polyploidy - 156(140-188)<6n>XXXX, -X, -X - multiple copies of: i(1p), i(1q), i(3p), i(6p), i(9q), add(5)(p15), add(16)(q23), i(mar) - 1-3 dmin/rings in 40% cells - isochromosome formation previously reported in thyroid tumors |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV +, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
