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| Cell line: | DMBM-2 |
| DSMZ no.: | ACC 269 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | macrophages |
| Origin: | established from the bone marrow of a C3H/HeJ mouse |
| Reference(s): | 14778 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing in aggregates, mostly loosely adherent, some cells have long processes; image; image; image |
| Medium: | 80% RPMI 1640 + 20% horse serum + 1 mM sodium pyruvate + 1 mM non-essential amino acids + 1.25 µg/ml vitamin B12 + 75 µM alpha-thioglycerin + 2 mM L-glutamine + 2.5% 10x L-929 supernatant (DSM ACC 2) |
| Subculture: | split 1:10 every 4-6 days detaching the cells by flushing the adherent cells with medium through a 25G needle (do not try to desaggregate cells by passing through the needle); cells grow best in Corning round plates (100 x 20 mm) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 50 hours |
| Harvest: | cell harvest of about 3-5 x 106 cells/plate |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine near-diploid karyotype with 10% tetraploidy - 42(37-43)<2n> - centric fusion markers absent |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
