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Cell line: | B-CPAP |
DSMZ no.: | ACC 273 |
Species: | human (Homo sapiens) |
Cell type: | thyroid carcinoma |
Origin: | established from the tumor tissue of a 76-year-old woman with metastasizing papillary thyroid carcinoma in 1992 |
Reference(s): | 14524, 15201 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | spindle- or round-shaped cells growing adherently as monolayer; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:4 to 1:6 every 3-4 days using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 30 hours |
Harvest: | about 10 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near-diploid karyotype with hexaploid sideline - 70/71(69-72)<3n>XX/XXX, +5, -7, -8, +11, +12, -13, +15, +19, -22, +mar - i(1p), 1qh+, t(5;5)(p13;p15), add(10)(p11), add(12)(p11), del(17)(p11), del(17)(p11)dup(17)(q11q23), add(19)(q13) - resembles published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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