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Cell line: | 4H1-A7 |
DSMZ no.: | ACC 28 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | hybridoma (anti-canine adenovirus 2) |
Origin: | established by fusion of SP2/0-AG14 myeloma cells with BALB/c mouse spleen cells immunized with purified canine adenovirus type 2 (CAV-2, Manhattan strain); the secreted antibody (IgG2a) binds to and neutralizes CAV-2 and is type-specific |
Reference(s): | 15059, 15060 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | single cells in suspension; image; image; image; image; image |
Medium: | 92.5% DMEM (4.5 g/L glucose) + 5% horse serum + 2.5% h.i. FBS |
Subculture: | maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:2 every 2-3 days |
Incubation: | at 37 °C with 5% CO2 |
Harvest: | maximum density at 1.0-1.5 x 106 cells/ml |
Storage: | frozen with 75% medium, 10% horse serum, 5% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Cytogenetics: | murine hypertetraploid/pentaploid karyotype with 5% polyploidy - 91-98<4n> - 3 centric fusion markers and 1-3 dmin present in most cells |
Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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