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| Cell line: | PYS |
| DSMZ no.: | ACC 284 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | teratocarcinoma |
| Origin: | the transplantable tumor OTT-6050 arose from a 6-day-old embryo grafted into the testis of a strain 129 mouse; this solid subcutaneous tumor was minced in PBS and mice were injected intraperitoneally; ascites containing embryoid bodies developed and cell cultures were initiated from the embryoid bodies |
| Reference(s): | 14711 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent epithelial-like cells growing as monolayers; image; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS |
| Subculture: | split confluent culture 1:5 to 1:10 every 2-3 days using trypsin/EDTA; seed out at ca. 2-3 x 10 cells/80 cm2 |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 24 hours |
| Harvest: | cell harvest of ca. 15 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine hypotetraploid karyotype - 70(66-72)<4n> - 7-11 centric fusion markers present |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
