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| Cell line: | P-388D1(IL-1) |
| DSMZ no.: | ACC 288 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | monocytes-macrophages |
| Origin: | established from the ascites of a DBA/2 mouse with a lymphoid neoplasm induced by methylcholanthrene and subsequently enriched for adherent (monocyte-macrophage) cells by selection (yielding this IL1-producing subclone); reported to produce IL-1 after stimulation with LPS or PMA, to phagocytize latex particles and to synthesize lysozyme |
| Reference(s): | 14436, 14687, 15131 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent elliptical and round loosely adherent cells growing in monolayers; image; image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% horse serum + 3.5 g/L glucose |
| Subculture: | seed out at ca. 1-3 x 106 cells/175cm2 flask; split confluent culture 1:3 to 1:6 every 3-6 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30-60 hours (according to density) |
| Harvest: | cell harvest of ca. 25 x 106 cells/175cm2 flask |
| Storage: | frozen with 70% medium, 15% horse serum, 5% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine hypertriploid karyotype with 8% polyploidy - 61(57-65)<3n> - 5-8 centric fusion markers |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
