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| Cell line: | RPMI-8402 |
| DSMZ no.: | ACC 290 |
| Species: | human (Homo sapiens) |
| Cell type: | T cell acute lymphoblastic leukemia |
| Origin: | established from the peripheral blood of a 16-year-old woman with acute lymphoblastic leukemia (ALL) in 1972. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
| Reference(s): | 14619, 15152, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly or in small clumps in suspension; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at about 0.5 - 0.7 x 106 cells/ml; maintain between 0.3 - 1.5 x 106 cells/ml;
split ratio of 1:3 to 1:6 once or twice a week |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 48 hours |
| Harvest: | saturation density at about 1-1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays |
| Immunology: | CD2-, CD3-, CD4-, CD5+, CD6(+), CD7(+), CD8-, CD13-, CD19-, CD34+, TCRalpha/beta-, TCRgamma/delta-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypotetraploid karyotype with 2% polyploidy - 90(79-91)<4n>XXX, -X, +3, +3, -10, -13, -14, +15, -18, -20, +2mar, dup(4)(q13q23)x2, del(6)(q14q22)x2, t(11;14)(p15;q11)x2, add(15)(p13) - sideline with der(1)t(1;9)(p35/36;q11), add(13)(q34) - carries t(11;14) with LMO1-TRD (LMO1-TCRD) rearrangement and cryptic del(1)(p32) effecting STIL-TAL1 (SIL-TAL1) fusion, resembles published karyotypes |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
