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| Cell line: | BA/F3 |
| DSMZ no.: | ACC 300 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | pro B cells |
| Origin: | IL-3 dependent murine pro B cell line; an original publication describing establishment of BA/F3 does not exist; probably a subclone of a first described Ea3 cell line. Performing May-Gruenwald-Giemsa staining provides evidence that the cells are indeed from a lymphatic background, however cells were apparently not derived from BALB/c, but from C3H mouse. Related publications see references |
| Reference(s): | 14829, 14830, 40553 |
| Biosafety level: | 1 |
| Risk assessment: | The German Central Committee on Biological Safety (ZKBS) classified this cell line risk group 2 when the cells are used in terms of genetic engineering. |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | mostly single, round (some polymorph) cells in suspension (or occasionally in clumps); image; image; image; image; image |
| Medium: | 95% RPMI 1640 + 5-% h.i. FBS + 10 ng/ml mouse IL-3 (or 10% vol conditioned medium of WEHI-3B cell line, DSMZ ACC 26) |
| Subculture: | seed out at about 1-3 x 105 cells/ml; split saturated culture 1:10 every 3 days; at high cell density (>2 x 106 cells/ml), a cytokine-independent subclone may grow out relatively quickly. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus, the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 20 hours |
| Harvest: | maximal density of about 2.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD11b (+), F4/80 (+); image |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine near diploid karyotype with 33% polyploidy - 40(36-42)<2n> |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
