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Cell line: | HC-1 |
DSMZ no.: | ACC 301 |
Species: | human (Homo sapiens) |
Cell type: | hairy cell leukemia |
Origin: | spontaneously immortalized peripheral blood leukocytes from a 56-year-old white man with hairy cell leukemia (HCL) at diagnosis; cells were described in the literature to grow in soft agar, to be tumorigenic in irradiated nude mice and to be negative for the HCL-associated mutation BRAF V600E. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
Reference(s): | 14931, 16917, 18187 |
Biosafety level: | 2 |
Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was detected by western blot after stimulating the cells with phorbol ester / sodium butyrate. The infection was classified as potentially lytic associated with a possible production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
Permissions and restrictions: | A, B |
| DSMZ Cell Culture Data: |
Morphology: | lymphoblastoid cells growing as single cells or in clumps, in suspension; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS |
Subculture: | split saturated culture 1:2 to 1:5 every 2-4 days; maintain at 0.3-0.6 x 106 cells/ml; seed out at ca. 0.5 x 106 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 45 hours |
Harvest: | saturation density of ca. 1.0-1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near-tetraploid karyotype with 3% polyploidy - 92(89-92)<4n>XXYY, add(2)(p13->23) - differs from published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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