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Cell line: | SK-MEL-1 |
DSMZ no.: | ACC 303 |
Species: | human (Homo sapiens) |
Cell type: | melanoma |
Origin: | established from the lymph node metastasis (thoracic duct) of a 29-year-old man with primary melanoma in 1966; cells were described in the literature to produce pigment and to lead to pigment-producing tumors upon heterotransplantation into hamster |
Reference(s): | 14810 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | mostly round cells growing singly or in clumps (occasional giant, polynucleated cells) in suspension; image; image |
Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
Subculture: | maintain between 0.1-0.3 x 106 cells/ml; seed out at 0.2 x 106 cells/ml; due to rapid acidification of the medium, spent culture medium should be exchanged frequently; a cell density of 0.5 x 106 cells/ml should not be exceeded |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 100 hours |
Harvest: | ca. 0.5-1.0 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypertriploid karyotype with 4% polyploidy - 78(72-79)<3n>XXYY, +X/Y, +2, +4, -5, +6, +7, +11, -12, -13, -13, +15, -17, +19, -21, +6mar - add(2)(p24), add(3)(q11), i(4q)x2, del(6)(q22), add(6)(q27), add(7)(q35)x2, del(9)(p23)x2, add(10)(p13), del(11)(p15), del(12)(q24), add(14)(p11), add(14)(q32), i(17q), add(17)(p12), add(19)(q13), del(?22)(q11) - carries pair of large submetacentric markers - matches published karyotype |
Molec. Genetics: | Genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation |
Viruses: | ELISA: reverse transcriptase negative: PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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