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| Cell line: | RBL-2H3 |
| DSMZ no.: | ACC 312 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | basophilic leukemia |
| Origin: | the rat basophlic leukemia (RBL) line was originally obtained by Eccleston et al. and was maintained by serial passage in neonatal Wistar rats; four cell lines (named RBL I-IV) were started from this tumor between 1973 and 1975; the subline RBL-IV HR+ was derived from RBL-IV; further subcloning established the cell line RBL-2H3; cells are described in the literature to release histamine as an IgE-mediated reaction |
| Reference(s): | 14372, 14696, 14500, 14613 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent, fibroblast-like cells growing mainly as monolayer and in a small extend as suspension culture; image; image |
| Medium: | 70% MEM (with Earle's salts) + 20% RPMI 1640 + 10% h.i. FBS; a higher proportion of RPMI 1640 may increase proliferation rate, but decreases histamine production |
| Subculture: | split culture before confluency 1:5 every 3 days using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2 |
| Incubation: | at 37 °C (some researchers favour cultivation with CO2) |
| Doubling time: | ca. 40--60 hours |
| Harvest: | cell harvest of ca. 15-20 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | histamine release confirmed by depositor |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Cytogenetics: | rat hypertriploid karyotype - 68(62-72)<3n> - carries large metacentric marker |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
