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| Cell line: | P-19 |
| DSMZ no.: | ACC 316 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | embryonal carcinoma |
| Origin: | established from the teratocarcinoma induced in a C3H/He strain mouse |
| Reference(s): | 14753, 14905 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid cells growing as adherent monolayer; image; image; image |
| Medium: | 80-90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10-20% h.i. FBS |
| Subculture: | split confluent culture 1:10 to 1:20 once or twice a week using trypsin/EDTA; seed out at ca. 0.3-0.4 x 105 cells/cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 2-3 days |
| Harvest: | cell harvest of max. 0.5 x 106 cells/cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine near-diploid karyotype with 16% polyploidy - 40(31-44)<2n> - one centric fusion marker - resembles published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
