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| Cell line: | SK-MEL-3 |
| DSMZ no.: | ACC 321 |
| Species: | human (Homo sapiens) |
| Cell type: | melanoma |
| Origin: | established from the lymph node metastasis of a malignant melanoma from a 42-year-old woman |
| Reference(s): | 15135 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent epithelial cells growing as monolayer; image; image; image |
| Medium: | 90% McCoy's 5A + 10% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2;
split confuent culture 1:3 to 1:4 every 3-4 days using trypsin/EDTA; |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 40-50 hours |
| Harvest: | cell harvest of ca. 15 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near-tetraploid karyotype with 3% polyploidy - 92(80-93)<4n>XX, -X, -X, -6, +7, +7, +7, -10, -10, -16, -16, +20, +20, +20, +20, +22, dup(7)(p15p22)x2, der(9)t(7;9)(p11;p21)dup(7)(p15p22)x2 - sideline with add(2)(p23) marker - resembles published karyotype |
| Molec. Genetics: | Genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
