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| Cell line: | VA-ES-BJ |
| DSMZ no.: | ACC 328 |
| Species: | human (Homo sapiens) |
| Cell type: | epithelioid sarcoma |
| Origin: | established from the bone marrow metastasis of an epithelioid sarcoma (epidural tumor) of a 41-year-old man following unsuccessful chemotherapy in 1991; cells were described in the literature to grow rapidly after heterotransplantation into nude mice, to secrete functional GM-CSF and to be positive for AE-1, AE-3, vimentin and epithelial membrane antigen |
| Reference(s): | 15138 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent cells growing in monolayers - epitheloid, partly fibroblastoid large cells; image; image; image |
| Medium: | 80% DMEM + 10-20% h.i. FBS + 1% non-essential amino acids + 2 mM L-glutamine |
| Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:2 to 1:5 once or twice a week |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 60-90 hours |
| Harvest: | cell harvest of max. 1.5 x 105 cells/cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypotriploid karyotype with 13% polyploidy - 68(65-70)<3n>XX, -Y, -3, -4, +6, +7, +7, -11, -12, +13, -14, -15, +17, +20, -22, add(1)(p32-34), add(1)(q43)x2, add(9)(q34)x3, del(22)(q13.2) - matches published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
