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| Cell line: | VERO-B4 |
| DSMZ no.: | ACC 33 |
| Species: | monkey - African green monkey (Chlorocebus aethiops (Cercopithecus aethiops)) |
| Cell type: | kidney |
| Origin: | established from the kidney of a normal adult African green monkey in Japan in 1962; cells are susceptible to a number of viruses; used in virus replication studies and plaque assays, and as indicator cell line for mycoplasma testing |
| Reference(s): | 15165 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A, E |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent-elongated, fibroblast-like cells in monolayers; image; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.5-1.0 x 106 cells/80 cm2; split confluent culture 1:3 to 1:10 using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 25 hours |
| Harvest: | cell harvest of ca. 20 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Chlorocebus aethiops |
| Cytogenetics: | simian rearranged flat-moded hypodiploid karyotype with 12% polypoidy - 54(48-61)<2n>X - VERO-B4 markers present |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
