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Cell line: | VERO-B4 |
DSMZ no.: | ACC 33 |
Species: | monkey - African green monkey (Chlorocebus aethiops (Cercopithecus aethiops)) |
Cell type: | kidney |
Origin: | established from the kidney of a normal adult African green monkey in Japan in 1962; cells are susceptible to a number of viruses; used in virus replication studies and plaque assays, and as indicator cell line for mycoplasma testing |
Reference(s): | 15165 |
Biosafety level: | 1 |
Permissions and restrictions: | A, E |
| DSMZ Cell Culture Data: |
Morphology: | adherent-elongated, fibroblast-like cells in monolayers; image; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.5-1.0 x 106 cells/80 cm2; split confluent culture 1:3 to 1:10 using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 25 hours |
Harvest: | cell harvest of ca. 20 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Chlorocebus aethiops |
Cytogenetics: | simian rearranged flat-moded hypodiploid karyotype with 12% polypoidy - 54(48-61)<2n>X - VERO-B4 markers present |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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