| | |
|---|
| Cell line: | MHEC5-T |
| DSMZ no.: | ACC 336 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | heart vascular endothelial cells |
| Origin: | spontaneous malignant transformation after homotransplanting MHEC5 NMRI mouse myocardial cells (2-weeks-old), thus forming hemangioendothelioma; cells produce tumors upon injection into histocompatible mice |
| Reference(s): | 14854 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent, epithelial-like cells growing as monolayers; image; image; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture: | seed out ca. 1 x 106 cells in a 80cm2 flask;
split confluent culture 1:3 to 1:6 every 3-6 days; use of trypsin/EDTA is not necessary (cells can be detached by tapping the flask or repeated pipetting); however, this results in cell clumps which are difficult to disperse; for single cells the use of trypsin/EDTA is recommended |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | cell harvest of ca. 5 x 106 cells/80cm2 flask |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine flat-moded hexaploid karyotype - 120(96-122)<6n> - 2-3r/dmin and 3 centric fusion markers |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
