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| Cell line: | DEL |
| DSMZ no.: | ACC 338 |
| Species: | human (Homo sapiens) |
| Cell type: | malignant histiocytosis |
| Origin: | established from the pleural effusion of a 12-year-old boy with malignant histiocytosis at diagnosis in 1987 (problably anaplastic large cell lymphoma, ALCL); initially established by serial passage in nude mice; cells carry the NPM1-ALK (NPM-ALK) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
| Reference(s): | 14370, 14485, 14492, 14464, 14737, 15185, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | grows often in clusters in suspension, most cells are polygonal (some cells are round); image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 0.4 x 106 cells/ml; split saturated culture 1:3 to 1:5 every 2-3 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | maximal density at ca. 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD5 -, CD6 -, CD7 -, CD8 -, CD10 -, CD14 -, CD19 -, CD25 +, CD30 +, CD34 -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human biclonal, hyperdiploid/hypertriploid, karyotype with 6.7% polyploidy - 47(44-49)<2n>XY, +5, +5, +6, -8, +mar, der(5)t(5;6)(q35;p21), t(5;6)(q35;p21), add(10)(q23-24), der(13)t(1;13)(q32;p11)t(1;13)(q21;q34), add(16)(q23), add(19)(p13), 33% - 74(74-77)<3n>XXY, +1, +3, +5, +5, +6, +7, -10, +13, -18, +20, t(5;6)(q35;p21)x2, add(10)(q24)x1-2, der(13)t(1;13)(q32;p11)t(1;13)(q21;q34)x2, add(16)(q23), add(19)(p13), 60% - matches published karyotype, t(2;5) not detectible cytogenetically |
| Molec. Genetics: | expression of NPM1-ALK (NPM-ALK) fusion gene was detected by RT-PCR analysis |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV +, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
