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| Cell line: | M3E3/C3 |
| DSMZ no.: | ACC 340 |
| Species: | Syrian hamster (golden hamster) (Mesocricetus auratus) |
| Cell type: | fetal lung |
| Origin: | established from the lung of a Syrian golden hamster fetus on day 15 after gestation in 1981; primary culture cells were treated with hydroxy-proline; cells were described as premature epithelial cells with the ability to differentiate into mucus-producing pulmonary cells |
| Reference(s): | 14511 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like cells growing in monolayers; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS + 1 mM sodium pyruvate |
| Subculture: | split confluent culture 1:3 to 1:6 every 3-4 days using trypsin/EDTA; seed out at 1-2 x 106 cells/80 cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 40 hours |
| Harvest: | cell harvest of ca. 8 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mesocricetus auratus species. |
| Cytogenetics: | near-diploid Syrian hamster karyotype with 10% polyploidy - 43-47<2n> |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
