| |
---|
Cell line: | 5637 |
DSMZ no.: | ACC 35 |
Species: | human (Homo sapiens) |
Cell type: | urinary bladder carcinoma |
Origin: | established from the primary bladder carcinoma of a 68-year-old man in 1974; cells are known to produce several growth factors (e.g. SCF, IL-1, IL-6, G-CSF, GM-CSF, etc.) |
Reference(s): | 15203, 14539, 15057, 14643, 14867 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epithelial-like adherent cells; image; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent cultures 1:4 to 1:5 using trypsin/EDTA (cells should be incubated for 5 to 10 min with trypsin/EDTA at 37 °C) |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 24 hours |
Harvest: | about 8-10 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | original contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypotriploid karyotype with 17% polyploidy - 62(47-64)<3n>XX, -Y, -4, -5, -10, -13, -13, -15, -18, -19, -21, -21, +4mar, der(1)t(1;?)(p21;?), der(3)t(3;?)(p22;?), del(6)(q23), der(9)t(9;?)(p12;?), der(11)t(5;11)(q14;p12), ?der(12)t(12;?)(q14;?), i(13q), i(14q), der(17)t(17;?)(p11/12;?), del(22)(q13) - carries large submetacentric marker with q arm resembling tandem dup(7) - 9p12 is a recurrent breakpoint in bladder carcinoma |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
| Print data sheet |