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Cell line: | LOVO |
DSMZ no.: | ACC 350 |
Species: | human (Homo sapiens) |
Cell type: | colon adenocarcinoma |
Origin: | established from the metastatic nodule resected from a 56-year-old man with colon adenocarcinoma in 1972; cells were described in the literature to produce CEA |
Reference(s): | 14468, 14469, 14470 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent, epithelial-like cells growing in monolayers; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 2-4 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 twice a week using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 48 hours |
Harvest: | cell harvest of ca. 35 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype with 16% polyploidy - 48/49<2n>XY, +7, +1-2mar, t(2;12)(q21;p11) - +7 recurrent in colon carcinoma - 1-2 small satellited markers present - resembles published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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