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Cell line: | SK-MES-1 |
DSMZ no.: | ACC 353 |
Species: | human (Homo sapiens) |
Cell type: | lung squamous cell carcinoma |
Origin: | established from the pleural effusion of a 65-year-old man with lung squamous cell carcinoma in 1970; listed in the CCLE (ref 18232) |
Reference(s): | 15135, 18232, 40405 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epithelial-like adherent cells growing as monolayers; image |
Medium: | 80-90% DMEM + 10-20% h.i. FBS |
Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture about 1:3 to 1:5 every 3-4 days using trypsin/EDTA |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 50 hours |
Harvest: | cell harvest of ca. 15 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hyperdiploid karyotype with 44% polyploidy - 54-60<2n>X, -Y, +1, +1, -2, +4, +5, +7, +7, +11, +13, +19, +22, +5mar, add(X)(p21), i(1q), del(1)(q12)x1-2, add(2)(q37), del(3)(p22p26), add(4)(q32), der(5)t(5;6)(p15;p11), del(5)(q13), add(7)(q11;abr), add(11)(q2)x2, add(13)(p12)x1-2, add(13)(p12), add(15)(p12), der(16)t(8;16)(q12;q23), del(17)(p11), add(19)(p13)x2-3 - sideline with additional der(7;8)(q10;p10), add(22)(q13), der(11)dup(11)(q13q22) - carries 3p-, 5q-, +7, 17p- associated with NSCLC - matches published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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