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| Cell line: | MHH-PREB-1 |
| DSMZ no.: | ACC 354 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell lymphoma |
| Origin: | established from the lymph node of a 5-year-old boy with B cell lymphoblastic non-Hodgkin lymphoma at diagnosis in 1994 |
| Reference(s): | not published |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly or in clusters in suspension; image; image; image; image; image; image |
| Medium: | 90-92.5% RPMI 1640 + 7.5-10% h.i. FBS |
| Subculture: | seed out at ca. 0.5-1.0 x 105 cells/ml; maintain at 0.5-2.0 x 106 cells/ml; split 1:10 to 1:20 twice a week |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 20-40 hours |
| Harvest: | cell harvest of ca. 2.5 x 106/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays |
| Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD138 -, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 12% polyploidy - 48<2n>XYY, +21, ?ins(2;2)(q21;p15p22), t(8;14)(q24;q32) |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
