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| Cell line: | HCT-15 |
| DSMZ no.: | ACC 357 |
| Species: | human (Homo sapiens) |
| Cell type: | colon adenocarcinoma |
| Origin: | established from a colorectal adenocarcinoma; HCT-15 is a sister cell line of the cell line DLD-1 (DSMZ ACC 278) |
| Reference(s): | 18260, 14456, 15218 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent epithelial-like cells growing as monolayer with contact inhibition; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out 2 x 106 cells in a 80 cm2 flask; split confluent culture 1:5 to 1:10 every 3-5 days using trypsin/EDTA; maximal density at ca. 50 x 106 cells/175 cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 20-25 hours |
| Harvest: | harvest of ca. 25 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to sister cell line DLD-1 (ACC 278) |
| Cytogenetics: | human pseudodiploid karyotype with 16% polyploidy - 46<2n>XYq+, t(8;17) (p23;q12/21), inv(11)(p14.2;q13) - stable karyotypic differences cf DLD-1 - matches published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
