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Cell line: | CAL-54 |
DSMZ no.: | ACC 365 |
Species: | human (Homo sapiens) |
Cell type: | kidney carcinoma |
Origin: | established from the malignant pleural effusion of a 75-year-old man with kidney carcinoma in 1987 |
Reference(s): | 14566 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | variously shaped, adherent cells growing in monolayers |
Medium: | 85% DMEM + 15% h.i. FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 0.4 µg/ml hydrocortisone + 10 ng/ml EGF |
Subculture: | split nearly confluent culture 1:2 to 1:3 about once a week using trypsin/EDTA (it may be difficult to detach the cells from the flask - use double quantity of trypsin/EDTA for ca. 15 min); seed out at ca. 2 x 106 cells/75 cm2 |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 72 hours |
Harvest: | cell harvest of ca. 8 x 106 cells/75 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype with 12% polyploidy - 52(52-53)<2n>X, -Y, +2, +3, +7, +7, +8, +12, -13, +15, +16, +17, del(3)(p21), del(5)(p14.2p15.2), dup(7)(q11q22), add(14)(p12) - del(3p), gain of ch 7 and loss of Y typical of renal cell carcinoma |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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