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variously shaped, adherent cells growing in monolayers; image; image
85% Dulbecco's MEM + 15% h.i. FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 0.4 µg/ml hydrocortisone + 10 ng/ml EGF
split nearly confluent culture 1:2 to 1:3 about once a week using trypsin/EDTA (it may be difficult to detach the cells from the flask - use double quantity of trypsin/EDTA for ca. 15 min); seed out at ca. 2 x 106 cells/75 cm2
at 37 °C with 5% CO2
ca. 72 hours
cell harvest of ca. 8 x 106 cells/75 cm2
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays
To inquire about expression of EpCAM and intermediate filaments, contact email@example.com.
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
human hyperdiploid karyotype with 12% polyploidy - 52(52-53)<2n>X, -Y, +2, +3, +7, +7, +8, +12, -13, +15, +16, +17, del(3)(p21), del(5)(p14.2p15.2), dup(7)(q11q22), add(14)(p12) - del(3p), gain of ch 7 and loss of Y typical of renal cell carcinoma