| |
---|
Cell line: | BFTC-909 |
DSMZ no.: | ACC 367 |
Species: | human (Homo sapiens) |
Cell type: | kidney transitional cell carcinoma |
Origin: | established from the sarcomatoid component of a grade III transitional cell carcinoma of the renal pelvis of a 64-year-old man in 1990; patient lived in a blackfoot disease endemic area in Taiwan probably related to TCC |
Reference(s): | 15028, 14430, 18168 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epithelial-like (spindle) cells growing in monolayers; cells contain considerable amounts of small, dark granules; image |
Medium: | 80-90% DMEM + 10-20% h.i. FBS |
Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent cultures 1:3 to 1:5 once or twice a week using trypsin/EDTA |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 50 hours |
Harvest: | cell harvest of ca. 3-5 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hypertriplod karyotype with 32% polyploidy - 66-79<3n>XX, -Y, +1, +3, +5, +7, -9, -11, +12, +17, -18, +19, +20, +21, +mar, add(1)(p34)x2, del(2)(p16), +dic(2;9)(q25;p22)x2, add(3)(p24)x2, add(6)(p23)x2, i(7p)x2, i(8q)x2, del(11)(q?22q23), add(12)(q24), add(19)(q13)x2 - 2-4 dmin present in most cells - gain of ch 7 and deletions of 9p typical in TCC - matches published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
| Print data sheet |