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Cell line: | KYSE-150 |
DSMZ no.: | ACC 375 |
Species: | human (Homo sapiens) |
Cell type: | esophageal squamous cell carcinoma |
Origin: | established from the poorly differentiated squamous cell carcinoma resected from upper (cervical) esophagus of a 49-year-old woman after receiving radiotherapy (tumor was invading contiguous structures); described as carrying amplified oncogenes, c-erb-B (8x) and cyclin D1 (4x) and producing tumors in nude mice; extensively characterized cell line (see cellosaurus), listed in CCLE (ref 18232) |
Reference(s): | 14646, 14954, 14955, 15006, 18232 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epitheloid cells growing as adherent monolayer; image; image |
Medium: | 49% RPMI 1640 + 49% Ham's F12 + 2% h.i. FBS |
Subculture: | seed out at ca. 1 x 106 cells/175 cm2; split confluent culture up to 1:20 every 3-4 days using trypsin/EDTA; cells grow very fast, a FBS amount around 2-5% is sufficient |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | below 25 hours (even less when serum amount is increased) |
Harvest: | cell harvest of ca. 15 x 106 cells in a 175cm2 flask |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypertriploid karyotype with 12% polyploidy - 70(68-75)<3n>XderX, -X, +1, -3, -4, -8, -9, +11, -14, -15, -16, -18, -21, +12mar (4xbisat), add(X)(q27), inv(1)(p34q32), del(1)(p32), del(1)(q11), del(7)(q32), del(8)(p21), i(9q), add(9)(q34), add(11)(p11), der(11)add(11)(p1?)add(11)(q24), del(1)(p11), del(12)(q21), del(15)(q23q25), add(19)(q13) |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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