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| Cell line: | TCC-SUP |
| DSMZ no.: | ACC 377 |
| Species: | human (Homo sapiens) |
| Cell type: | urinary bladder transitional cell carcinoma |
| Origin: | established from the tumor specimen resected from the urinary bladder transitional cell carcinoma (undifferentiated, grade IV) of a 67-year-old woman in 1974 (with therapy) |
| Reference(s): | 14803, 14579, 15063 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like adherent cells with smooth borders growing as monolayer; image; image; image |
| Medium: | 80-85% DMEM (4.5 g/L glucose) + 15-20% h.i. FBS |
| Subculture: | split confluent culture 1:5 to 1:10 every 3-4 days using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2 in 10 ml medium |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30-40 hours |
| Harvest: | cell harvest of ca. 6 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypotriploid karyotype with 8% polyploidy - 67(58-67)<3n>XX, -X, +1, -2, -4, +5, -9, -12, -18, -21, +1-2mar, der(1;21)(q10;q10), del(1)(q13), i(5p)x3, del(6)(q14), add(6)(p23)/der(6)t(3;6)(p22;p22), add(6)(p22/23), del(10)(p11), add(20)(q12), add(21)(p11/12) - i(5p) and rearrangements of chs 1 and 3p recurrent in bladder TCC - resembles published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
