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| Cell line: | KU-812 |
| DSMZ no.: | ACC 378 |
| Species: | human (Homo sapiens) |
| Cell type: | chronic myeloid leukemia in myeloid blast crisis |
| Origin: | established from the peripheral blood of a 38-year-old man with chronic myeloid leukemia (CML) in myeloid blast crisis; described in the literature to express basophilic features; carries t(9;22) leading to BCR-ABL1 e14-a2 (b3-a2) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
| Reference(s): | 14663, 14479, 14488, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single round cells growing in suspension; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | cells grow very slowly; seed out at ca. 0.5 x 106 cells/ml; after thawing, cells may have a lag phase of 1-2 weeks before reassuming vigorous growth; then, maintain at 0.4-0.8 x 106 cells/ml; split 1:2 every 3-4 days; viability may not exceed 80% |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 80-100 hours |
| Harvest: | maximum density of ca. 1.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD13 +, CD14 -, CD15 -, CD33 +, CD71 +, CD235a +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypotriploid karyotype with 8% polyploidy - 61(58-62)<3n>XYY, -2, -3, -5, +6, -7, +8, -10, -12, -16, -17, -18, +19, -20, t(9;22)(q34;q11)x2, i(11q), i(17q) - Ph (two copies) together with i(17q) and additional chs 8 and 19 - matches published karyotype |
| Molec. Genetics: | expression of fusion gene BCR-ABL e14-a2 (b3-a2) confirmed by RT-PCR |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
