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Cell line: | PF-382 |
DSMZ no.: | ACC 38 |
Species: | human (Homo sapiens) |
Cell type: | T cell leukemia |
Origin: | established from the pleural effusion of a 6-year-old girl with acute lymphoblastic leukemia (ALL) in second relapse |
Reference(s): | 14842 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | single, round to oval cells in suspension; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-2.0 x 106 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 70 hours |
Harvest: | maximal density of about 3-4 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | CD2 +, CD3 -, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 +, TCRalpha/beta -, TCRgamma/delta -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near-diploid karyotype with 9% near-tetraploid and 15% polyploid sidelines - 45/46(43-47)<2n>X/XX, -10, +14 - sideline with +add(?15)(p11), add(1)(p32) - identity confirmed |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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