| | |
|---|
| Cell line: | KYSE-270 |
| DSMZ no.: | ACC 380 |
| Species: | human (Homo sapiens) |
| Cell type: | esophageal squamous cell carcinoma |
| Origin: | derived from well differentiated invasive squamous cell carcinoma resected from middle intra-thoracic esophagus of a 79-year-old man prior to treatment (depth of invasion was not beyond the muscularis propria); described in the literature to carry TP53 mutation |
| Reference(s): | 14954, 14955, 15006, 14646, 18232 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid cells growing in monolayers; image; image; image; image; image |
| Medium: | 49% RPMI 1640 + 49% Ham's F12 + 2% h.i. FBS |
| Subculture: | seed out at ca. 0.5-2 x 106 cells/80 cm2; split confluent culture 1:5 every second day using trypsin/EDTA (incubation for 10-15 min) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 24 hours |
| Harvest: | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypotriploid karyotype with 16% polyploidy - 66(64-67)<3n>XX/XXX, +1, -2, +3, -5, -6, +7, +8, -9, -10, -15, -15, -17, -18, -19, -20, -22, +5mar (1bisat), ?del(X)(q25), add(1)(p1?), add(1)(p2?), i(1p), der(1)t(1;?)(?;2)(q13;?)(?;q11), add(2)(q37), del(2)(q23), add(3)(p2?6)x2, i(5p), add(5)(p15), del(7)(p11), add(8)(q24), add(16)(q24), add(18)(q23), add(19)(p13), add(20)(q12), add(21)(p11) |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
