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Cell line: | YAPC |
DSMZ no.: | ACC 382 |
Species: | human (Homo sapiens) |
Cell type: | pancreas carcinoma |
Origin: | established from ascites obtained during exploratory laparatomy of a 43-year-old man with pancreas carcinoma (terminal, no therapy) in 1993; cells were described as being dependent on autocrinely secreted IL-1alpha |
Reference(s): | 15070, 40710,40728 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | cobblestone-like adherent cells growing in monolayers; image; image |
Medium: | 85% RPMI 1640 + 15% h.i. FBS |
Subculture: | seed out at ca. 1-2 x 106 cells/ 175 cm2; split culture up to 1:10 every 4 to 6 days prior to confluence (as the monolayer detaches upon reaching confluency) using trypsin/EDTA (incubate with trypsin/EDTA for 10-20 min); optimal density is at about 5 x 106 cells/175 cm2; it is recommended to add 10% of the spent medium to any fresh culture; after thawing cells may have a lag time of 1-2 days before reassuming growth |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 50-60 hours |
Harvest: | cell harvest of ca. 2.0 x 107 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near-triploid karyotype with 8% polyploidy - 66-71<3n>XXYY, +3, +7, +8, +9, +10, +14, -17, -18, -19, -20, -22, -22, -22, +3mar, der(1)del(1)(q23-25)inv(1)(p11q21), der(1;5)(q10;q10), add(3)(p11)/del(3)(p11p21)x2, del(5)(p11), add(6)(p21), del(7)(q32), add(9)(p1?), der(9;15)(q10;q10), der(10)t(10;11)(p11;q13)x2, del(12)(p12), add(13)(p11)x2, add(13)(p11), inv(16)(p13q11.2), del(18)(q21), add(20)(p11)/der(20)t(1;20)(p34;p11) - loss of 1p, 3p, 9p, 18q associated with pancreas carcinoma - matches published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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