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| Cell line: | EPLC-272H |
| DSMZ no.: | ACC 383 |
| Species: | human (Homo sapiens) |
| Cell type: | epidermoid lung carcinoma |
| Origin: | established from a surgical lung specimen of a 57-year-old man who underwent surgery for undifferentiated squamous cell carcinoma of the lung (with having received prior radiation or chemotherapy) in 1986 |
| Reference(s): | 14384 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent epithelial-like cells (with sharp contours) growing adherently as monolayers; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:2 to 1:3 every 3-5 days using trypsin/EDTA (incubate for >10 min) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 60 hours |
| Harvest: | cell harvest of ca. 5-7 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypotriploid karyotype with 12% polyploidy - 66(59-70)<3n>XX, -Y, -4, +7, +8, -18, -19, -20, -21, +6mar, add(1)(p32), del(2)(q33), der(2;11)(q10;q10), add(3)(p25), i(5p), i(5q), add(6)(q11), add(7)(q33)x2-3, add(7)(p15), del(8)(p12-21), del(10)(p14)x1-2, add(11)(q24)x2, add(12)(p11-12), add(13)(q33), add(14)(p12), i(15q), der(17)t(3;17)(p11;p12), add(19)(q13), add(21)(p12), dmx2 - matches published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
