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Cell line: | EOL-1 |
DSMZ no.: | ACC 386 |
Species: | human (Homo sapiens) |
Cell type: | acute myeloid (eosinophilic) leukemia |
Origin: | established in 1984 at diagnosis from the peripheral blood of a 33-year-old man with acute myeloid (eosinophilic) leukemia (AML) following hypereosinophilic syndrome; we found that EOL-1 carries a KMT2A (MLL) partial tandem duplication; described in the literature to carry the fusion FIP1L1-PDGFRA. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
Reference(s): | 14912, 14751, 14868, 18187, 14439 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | (relatively small) round cells growing in suspension, singly or in small clusters; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.5 x 106 cells/ml; start culture with 20% FBS; maintain at 0.5-2.0 x 106 cells/ml; split 1:2 to 1:3 every 3-4 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 60 hours |
Harvest: | maximum density of ca. 2 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | CD3 -, CD4 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype with 7.5% polyploidy - 50(48-51)<2n>,XY, +4, +6, +8, +19, del(4)(q12)x2, del(9)(q22) - del(4) effects cryptic FIP1L1-PDGFRA fusion - matches published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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