| | |
|---|
| Cell line: | LCLC-97TM1 |
| DSMZ no.: | ACC 388 |
| Species: | human (Homo sapiens) |
| Cell type: | large cell lung carcinoma |
| Origin: | cell line represents the xenotransplant of a primary tumor which was obtained from a 44-year-old man with large cell lung carcinoma who did not have any prior therapy; listed in the CCLE (ref 18232) |
| Reference(s): | 14382, 18232 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent epitheloid cells, densely packed in islets, growing as monolayers; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 once or twice a week using trypsin/EDTA before reaching complete confluency; medium (culture supernatant) should be exchanged at least every second day (even with splitting the cells) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | >50 hours |
| Harvest: | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid/hypotriploid karyotype - 58(50-60)<3n>X, -X/Y, -X/Y, +1, +1, +5, +5, -7, -10, -11, +12, -13, -15, -15, -15, -17, -18, -18, -19, -20, -21, -21, -22, der(1;7)(q10;q10)x2, der(1;15)(p10;q10)x2, der(3;6)(q10;q10), der(5;18)(p10;p10), der(5;18)(q10;q10), der(6;10)(p10;q10), i(7p)x2, i(8p), i(8q)x2, der(11)t(1;11)(q11;q24), der(14)t(7;14)(q12;q32), der(14)t(7;14)(q12;q32)add(14)(p11), der(14)t(7;14)(q12;q32)t(14;21)(p10;q10) - carries an unprecedented cluster of whole-arm translocations - resembles published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
