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Cell line: | SUP-B15 |
DSMZ no.: | ACC 389 |
Species: | human (Homo sapiens) |
Cell type: | B cell precursor leukemia |
Origin: | established from the bone marrow of a 9-year-old boy with acute lymphoblastic leukemia (B cell precursor ALL) in second relapse in 1984; described in the literature to carry the ALL-variant (m-bcr) of the BCR-ABL1 fusion gene (e1-a2) |
Reference(s): | 14800, 14488 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | small round cells growing singly in suspension; image; image; image; image |
Medium: | 80% McCoy's 5A + 20% h.i. FBS |
Subculture: | seed out at ca. 1 x 106 cells/ml; it is advisable to culture the cells initially in 24-well-plates, cell growth may be slow during the first week after thawing; maintain at ca. 0.5-2.0 x106 cells/ml; split 1:2 to 1:3 about once or twice a week |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 60 hours |
Harvest: | cell harvest of ca. 1.5 x 106 cells/ml; maximum density of ca. 3 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | CD3 -, CD10 +, CD19 +, CD34 +, CD37 -, CD38 +, CD138 -, HLA-DR +, sm/cyIgG -, smIgM -, cyIgM +, sm/cykappa -, sm/cylambda -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human pseudodiploid karyotype - 46<2n>XY, der(1)t(1;1)(p11;q31), add(3)(q2?7), der(4)t(1;4)(p11;q35), t(9;22)(q34;q11), add(10)(q25), ?del(14)(q23q31), der(16)t(9;16)(q11;p13) - resembles published karyotype |
Molec. Genetics: | expression of fusion gene BCR-ABL1 (e1-a2) confirmed by RT-PCR |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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