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Cell line: | KU-19-19 |
DSMZ no.: | ACC 395 |
Species: | human (Homo sapiens) |
Cell type: | urinary bladder transitional cell carcinoma |
Origin: | established from the invasive transitional cell carcinoma of the bladder (grade 3, pT3b) of a 76-year-old man in 1993; described as secreting several hematopoietic cytokines and chorionic gonadotropin |
Reference(s): | 14987, 14998 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epithelial-like cells growing adherently in monolayers; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 once or twice a week using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 48 hours |
Harvest: | cell harvest of ca. 15 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypertriploid karyotype - 74(70-75)<3n>XXX, +1, +5, +6, -7, +9, -11, +12, +15, +16, -17, -18, +19, +21, -22, +2mar - add(1)(p32-34)/del(1)(p34), add(4)(q35)x1-2, del(5)(q14.2), add(6)(q11), del(11)(p14), add(12)(q24)/der(12)t(12;17)(q24;q12-22), add(19)(q13) - hypertriploid derivate of original hypotetraploid clone - 4q+ and 19q+ markers confirm identity |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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