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Cell line: | CTV-1 |
DSMZ no.: | ACC 40 |
Species: | human (Homo sapiens) |
Cell type: | T cell leukemia |
Origin: | allegedly established from the peripheral blood of a 40-year-old man with acute monoblastic leukemia (AML M5) at relapse in 1982 (according to original publication allegedly derived from a woman); however, recent new data suggest derivation from T-ALL (acute lymphoblastic leukemia): (1) T-ALL-associated gene expression signature (Andersson et al., Leukemia 19: 1042-1050, 2005); (2) own FISH analysis confirming t(1;7) effecting TAL1-TRB fusion; (3) own reanalysis of the immunoprofile |
Reference(s): | 14428, 14357 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round or multiformed cells, singly or clustered in suspension; image; image; image; image; image |
Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
Subculture: | seed out at ca. 0.8-1.0 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml;
split 1:2 to 1:4 every 3-4 days; after thawing, culture should first be started with 20% FBS and best in 24-well-plates |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 40 hours |
Harvest: | maximal density at about 1.0-1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | CD2 -, cyCD3 +, smCD3 -, CD4 -, CD5 -, CD6 (+), CD7 +, CD8 -, CD13 -, CD15 +, CD19 -, CD33 -, CD34 -, HLA-DR -, TCRalpha/beta -, TCRgamma/delta -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near tetraploid karyotype with 9% polyploidy - 92(89-94)<4n>XX/XXY/XXYY, -1, +6, +6, -17, t(1;7)(p34.2;q34)x2, del(3)(p21), i(6q)x2, t(12;16)(q24.32;q11)x2 |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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