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Cell line: | VM-CUB-1 |
DSMZ no.: | ACC 400 |
Species: | human (Homo sapiens) |
Cell type: | urinary bladder transitional cell carcinoma |
Origin: | established from the primary transitional cell carcinoma of the urinary bladder of a male patient; described in the literature to carry a p53 point mutation |
Reference(s): | 14579, 15063 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent epithelial-like cells growing as monolayers; image; image; image |
Medium: | 90% DMEM (4.5 g/L glucose) + 10% h.i. FBS |
Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 every 3-5 days using trypsin/EDTA |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 30-45 hours |
Harvest: | cell harvest of ca. 40 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypertriploid karyotype with 4% polyploidy - 80<3n>XX/XX?Y, +1, +1, +5, +6, +7, +9, -11, +15, -18, +19, +20, +22, +22, +7mar, del(1)(q21), del(1)(q11), der(1;6)(p10;q10)x2, add(3)(p21.3)x2, i(5p), der(6)t(1;6)(q11;q22), ?inv(7)(p15q35), i(9q), del(10)(p11), del(10)(q?22?24), add(12)(p11)x2, add(15)(p11), add(21)(p11) - sideline with i(5q) |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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