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| Cell line: | FDCP-Mix cl.A4 |
| DSMZ no.: | ACC 401 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | bone marrow |
| Origin: | established from the long-term bone marrow cultures of BDF1 mice; cells are mIL-3-dependent (FDCP means "Factor-Dependent Continuous cell lines, Paterson Laboratories" after the Paterson Institute, Manchester, UK); it is described that cells can be maintained in a primitive state or induced to differentiate |
| Reference(s): | 14977, 14978 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round, single cells in suspension, sometimes in small clusters; image; image; image |
| Medium: | 80% Iscove's MDM + 20% h.i. FBS + 10 ng/ml murine IL-3 (or 10-20% conditioned medium of cell line WEHI-3B, DSM ACC 26) |
| Subculture: | split saturated culture 1:2 every 3-4 days; seed out at ca. 1.0 x 106 cells/ml; after thawing, cells are initially very difficult to culture and viability may be initially rather low (20-30%): hence it is advisable to culture the cells first in 24-well-plates and to exchange the medium, adding fresh mIL-3, already about 5-8 hours after thawing and then daily. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 70-80 hours |
| Harvest: | maximum density at about 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine near-diploid karyotype with 10% polyploidy - 40(38-40)<2n> - fusion markers absent |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
