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| Cell line: | RED-1 |
| DSMZ no.: | ACC 407 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | erythroid leukemia |
| Origin: | established from a Rauscher murine leukemia virus-induced erythroid leukemia of a female DBA/2 mouse; cells were described in the literature to be responsive to erythropoietin (EPO) and to form benzidine-positive colonies upon stimulation with EPO or DMSO |
| Reference(s): | 14589 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round, single cells growing in suspension; image; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS |
| Subculture: | maintain at 0.5 x 106 cells/ml; split saturated culture at 1:4 to 1:5 every second day; seed out at ca. 0.5-1.0 x 106 cells/ml; viability immediately after thawing may be rather low (<30%), but cells recover quickly and viability will raise over the next few passages; maximal density at ca. 2-3 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 24 hours |
| Harvest: | cell harvest of ca. 1-2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine hypodiploid karyotype with 10% polyploidy - 39(38-40)<2n>, t(X;1)(q10;q10) - two centric-fusion markers present - resembles published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
