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| Cell line: | 639-V |
| DSMZ no.: | ACC 413 |
| Species: | human (Homo sapiens) |
| Cell type: | ureter transitional cell carcinoma |
| Origin: | established from the transitional cell carcinoma (small, grade III) removed from the left ureter of a 69-year-old man in 1973 |
| Reference(s): | 14506, 14505, 15063 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like cells growing adherently in monolayers; image; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/25 cm2; split confluent culture 1:5 to 1:10 every 3-4 days using trypsin/EDTA |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | cell harvest of ca. 6 x 106 cells/25 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 24% polyploidy - 55(50-59)<2n>XX, +1, +3, +5, +6, +8, +11, +12, +14, -15, +20, -21, add(1)(p?21), i(5p), del(6)(q13-14), i(7p), add(8)(p2?), add(11)(p11), del(12)(q21q24), add(18)(q23) - additional copies of 6q-, i(7p), 8p+, der(18) markers present in sidelines together with del(7)(p13-14) |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
