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| Cell line: | 647-V |
| DSMZ no.: | ACC 414 |
| Species: | human (Homo sapiens) |
| Cell type: | urothelial bladder carcinoma |
| Origin: | established from a male patient with a primary urothelial bladder carcinoma (malignant, grade 2) |
| Reference(s): | 14505, 15063, 14506 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent epithelial-like cells growing in monolayers; image; image |
| Medium: | 85% DMEM + 15% h.i. FBS + 2 mM L-glutamine |
| Subculture: | seed out at ca. 2 x 106 cells/80 cm2 in 10 ml medium; split confluent culture 1:4 to 1:8 every 3-4 days using trypsin/EDTA (wash twice with PBS before trypsination) |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | cell harvest of ca. 10-15 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hyperdiploid/hypotriploid karyotype with 12% polyploidy - 52-60<3n>XX, -X/Y, -2, -4, -6, -9, -10, -12, -12, -14, -15, -16, -17, -18, +5mar, del(1)(p32), del(2)(q23)/add(2)(q2?), add(3)(q27), add(3)(q1?), i(5p), i(6p), i(7p)x2, add(8)(q24), del(11)(q22q24), del(13)(q?11q?14), der(19)t(12;19)(q13;q13), add(21)(p11) - i(5p) associated with bladder cancer |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
