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| Cell line: | C7 |
| DSMZ no.: | ACC 416 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | macrophages |
| Origin: | established from the bone marrow of male adult 129/Sv mice (p53 gene knocked out); it is described that the differentiation potential of C7 cells to osteoclasts is maintained in the presence of M-CSF and is abolished irreversibly by the stimuli known to induce differentiation into other hematopoietic lineages |
| Reference(s): | 14771 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | macrophage-like adherent cells with processes; image; image |
| Medium: | 90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10% h.i. FBS + 1 ng/ml M-CSF (human or murine) |
| Subculture: | split confluent culture 1:2 to 1:4 every 4-6 days using trypsin/EDTA after washing twice with PBS; seed out at ca. 0.5 x 106 cells/80 cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 80-100 hours |
| Harvest: | cell harvest of ca. 2-3 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine hypertriploid karyotype with 20% polyploidy - 66(64-67)<3n> - no centric fusion markers present |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
