| |
---|
Cell line: | RT-112 |
DSMZ no.: | ACC 418 |
Species: | human (Homo sapiens) |
Cell type: | urinary bladder transitional cell carcinoma |
Origin: | established from the transitional cell carcinoma (histological grade G2) excised from a woman (age unknown) with untreated primary urinary bladder carcinoma in 1973; described in the literature to form tumors in nude mice |
Reference(s): | 14738, 14734, 14600, 18249 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent epitheloid cells growing as monolayer; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2; split culture prior to confluence 1:2 to 1:4 every 2-5 days using extended trypsin/EDTA treatment (5-10 min) |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 35 hours |
Harvest: | cell harvest of ca. 20-30 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype with 4% polyploidy - 47(44-47)<2n>XX/XXX, +X, +10, +11, +14, -17, +mar, add(2)(q3?2), del(3)(p14p24.1), i(4p), add(6)(q2?), i(8q), del(9)(p21p22), add(10)(p12), der(11)t(3;11)(q11-13;p15), add(17)(p1?1), add(18)(q11-22) - sideline with: idem, del(9)(p11), del(11)(q23), add(12)(q24.32), del(13)(q11q14), add(21)(p11) - resembles published karyotypes |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
| Print data sheet |