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cell line was established by fusion of mouse NS-1 cells with spleen from a BALB/c mouse immunized with enriched retinal cell membranes; described as secreting a mouse monoclonal antibody (IgM) reacting with the chicken dorsal retina cytoplasmic membranes (E12) and binding outer photoreceptor elements of adult vertebrate retina (inclusive chick, mouse, rat)
spheroidal cells growing singly in suspension, or occasionally loosely adherent
90% RPMI 1640 + 10% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement" for the first 1-2 weeks post-thawing)
maintain at ca. 0.1-0.3 x 106 cells/ml; split saturated culture 1:3 to 1:10 every 2-5 days (some cells attach to the surface of the culture flask, but can be detached by tapping the flask); seed out at ca. 0.3 x 106 cells/ml; a maximal density of 1.0 x 106 cells/ml should not be exceeded
at 37 °C with 5% CO2
ca. 30 hours
cell harvest of ca. 1.0 x 106 cells/ml
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
secretion of a IgM monoclonal antibody
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.