| | |
|---|
| Cell line: | RED-4 |
| DSMZ no.: | ACC 425 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | erythroid leukemia |
| Origin: | established from a Rauscher murine leukemia virus-induced erythroid leukemia of a DBA/2 mouse; in contrast to RED-1 (DSM ACC 407), cells were described in the literature to be unresponsive to erythropoietin (EPO) or DMSO |
| Reference(s): | 14589 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round, single cells growing in suspension; image; image; image |
| Medium: | 85-90% DMEM + 10-15% h.i. FBS |
| Subculture: | maintain at 0.5 x 106 cells/ml; split saturated culture at 1:2 to 1:3 every day; seed out at ca. 1.0 x 106 cells/ml; viability immediately after thawing may be rather low (<30%), but cells recover subsequently over the next week and viability will raise over the next few passages (spent medium should be exchanged with discarding cells); cells grow better in 24-well-plates; maximal density at ca. 1.0 x 106 cells/ml |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 24 hours |
| Harvest: | cell harvest of ca. 1.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine near diploid karyotype with 12.5% polyploidy - 39-42<2n>XX, Df(2E2F1) - 1-2 centric fusion markers present - resembles published karyotype |
| Viruses: | ELISA: reverse transcriptase positive |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
